How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

02 December 2020 9,424 5 View

Why CD4 Treg lose FoxP3 during expansion in vitro?

Hello everyone! I have the following question: I tried to expand CD4 Tregs in vitro to get enough material for WB or any other application. This is my first trial with them. So, I`ve got FoxP3+...

13 September 2020 6,458 6 View

How should I care of mice after sublethal irradiation?

Hello dear colleagues I plan to test the primary antiviral response of CD8 T-cells of different genotypes, WT and with total knockout of some gene. The problem is that the KO mice that I plan to...

22 July 2019 1,844 3 View

Do exist any approved to human (or, at least, near to be) anti-inflammatory drugs, alternatives to NSAIDs?

09 August 2017 2,919 7 View

Classifier robust against non-informative features?

I have two feature sets I evaluated using SVM on a two-class problem. The first set (the "good" one) provides acceptable results, while the second one is far behind. When I merge the two sets...

12 March 2014 161 3 View

How do you specify CPSET and NSET for history output?

The simulation, I am trying to bring about, is essentially related to a contact analysis between deformable and analytical rigid bodies, the interaction between them is defined by means of surface...

13 February 2014 1,142 1 View

Dear colleagues! Do you think that all the team roles according to the Belbin model* will remain unchanged in the future?

Perhaps, some will cease to be relevant and, conversely, new ones will arise? And if the changes are still there, then what and why? (*Belbin model =...

01 January 1970 9,973 4 View

Transition from academic to business career: mission impossible?

Dear Colleagues! As far as, from your point of view, it is possible (and justified) to implement the concept of Life Long Learning in a professional academic career by completely withdrawing from...

01 January 1970 4,724 15 View

The need to disseminate information about the results of their scientific activities for the researcher in modern conditions.

Dear Colleagues! Do you think a modern researcher should independently make efforts to spread awareness in the world around him about the results of his scientific activity? If so, how much will...

01 January 1970 9,054 2 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View