Hello Rajesh,

I think it's easier to find COI primers to do DNA barcoding on Animals. ITS2, usually, is used for Plants and Fungi, but I think it depends on which taxa you want to track. So, what kind of taxa do you want to study with this technique?

You can start from this: Article Use of ITS2 Region as the Universal DNA Barcode for Plants and Animals

it's an article about ITS2 for Plant and Animals. Maybe it was cited by someone that developed ITS2 "universal" primers.

Hope I was helpful in someway.

Thank you, your suggestion will be helpful for me....actually i want these ITS primers, COI and COX primer for DNA bar-coding of some Indian species tortoises.