How many mice are needed for long term potentiation?

As above, we are trying to do the Long-term potentiation test using mice hippocampal slices. How many mice are needed or how many slices are needed for LTP? Can anyone help me with that?

15 February 2020 7,073 3 View

Does anyone happen to know how to deal with kernel random matrix without using any expansion (i.e., Taylor, series, orthogonal or bi-orthogonal)?

The literature all depends on some kind of expansion. It is useful for global analysis but not for local.

28 January 2019 6,589 1 View

ARIMA, R square and stationary R square?

Dear all When ARIMA model was used to make the unstable time series data stable. There are many model to choose. which is the best one? Not only BIC value was shown, but also R square,...

24 December 2018 7,782 3 View

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

12 November 2018 3,755 4 View

How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

31 October 2017 3,663 3 View

HOW TO DO ATRP REACTIONS ON THE SURFACE OF SiO2 NANOPARTICLES?

I encounter two questions during SI-ATRP. My experiment route is shown as follows. First ATRP reaction, MSN-Br(100mg), GMA( Glycidyl methacrylate, 1.5mmol),...

23 December 2016 3,066 3 View

Are deep neural networks practical to problems with low-dimensional input data(e.g. under 10 dimensions) ?

Deep neural network has been proved that efficient in imaging, text and voice data. Are they work good in processing low dimensional data ? (One example, using the DNN to analysis mechanical...

19 May 2016 3,367 3 View

How to do protein purification by intein tag/chitin beads in mammalian cells?

17 April 2016 1,304 2 View

What's the difference between the polarization direction, maximum radiation direction and direction of the electric field distribution?

10 February 2016 7,955 2 View

Is measuring Bradykinin levels in plasma possible?

Hi, does anyone have the experience in measuring bradykinin (BK) level in samples (plasma, medium etc). Since BK is easily degraded to its metabolites in vivo, I think elisa kits that only react...

29 December 2015 6,350 2 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

What is difference between In-line transmission (%) and Total transmission (%) value of optically transparent Ceramics?

I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...

20 February 2021 9,405 6 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

What is role of toluene and stearic acid in ball milling for powder ? In what weight percent we should add this to powder?

I am doing ball milling operation of Aluminum and silicon carbide powder. I am pretty confused about what should be added as process control agent ? what is role of toluene as PCA and stearic...

12 February 2021 5,205 3 View