What all thing we should keep in mind while selecting a solvent for hydrothermal reaction in a 50 ml autoclave?

I am going to use a 50 ml teflon lined autoclave for doing hydrothermal synthesis and I am deeply concerned about its safety. The pressure limit inside the autoclave is 3MPa and temperature...

14 February 2018 7,313 3 View

Are there any distinct morphological characters by which Anopheles subpictus species B can be distinguished from other sibling speices?

The available morphological character for distinguishing adults of species B is the lengths of apical white band and subapical dark band of female palpi. However, there are reports of variations...

25 January 2017 1,400 4 View

Is it possible to isolate/detect Japanese Encephalitis virus from Bird droppings?

Migratory birds are likely to transport JE viruses from an endemic area to a non-endemic area. Screening their blood would be the best method to detect JE virus in them. However, it is not...

01 April 2015 8,156 12 View

What is the current status of the Zika virus in Asia?

26 February 2014 7,707 3 View

Does anybody know various theories on the origin and evolution of insects?

In our entomology syllabus one of the components is origin and evolution of insects-various theories. Can anybody suggest a suitable book/paper dealing with this issue?

25 November 2013 4,229 3 View

Can anybody identify the species?

It was found inside an unopened packet of Killer Zone trilogy CD for PS3 brought from the US.

22 November 2013 9,553 14 View

T-test as a statistic for the analysis of fMRI data - can anyone help?

Why are t-tests preferred over other statistical tests for the analysis of fMRI data?

05 October 2013 8,594 8 View

How does matter get its mass ?

By interaction with Higgs field?

08 August 2012 5,331 2 View

What is the total number direct product groups of the form Zn1*Zn2*..... isomorphic to Zn? Here n1*n2*.....=n.

Every cyclic group is isomorphic to Zn. Now what is the total number of isomorphic direct product groups of the form Zn1*Zn2*Zn3*........Where n1*n2*n3*.....=n. It is known that (Zp*Zq) is cyclic...

16 November 2011 5,242 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View