Sabyasachi Dash

4 Questions 21 Answers 0 Followers

Questions related from Sabyasachi Dash

How can I define RNA stability/saturation versus induced protein expression for any given gene?

assuming a case where we see induction in protein expression levels of any gene X due to nutrient stress but the transcript levels getting saturated and plateaued after a minor induction with...

15 September 2015 567 3 View

Can I use indirect ELISA using bacterial cells as antigens to see expression of certain surface proteins?

I want to coat bacterial cells at a cell number of 10 (^8). I need help in regards to how to design the strategy for coating of my cells, assuming that each well of the 96 well plate will have...

30 July 2014 3,221 14 View

Can a promoter amplify genes which are homologous?

For example: Cold shock proteins CspA, CspB, CspG etc. are known to be homologous and are cold inducible proteins. Can we engineer a vector that would contain the promoter of, let's say, CspA but...

03 June 2014 9,528 2 View

Can Chloroform Lyse gram positive bacteria, such as Bacillus? And will it affect bacteriophages if co-cultured with bacillus?

In a situation where there is a host specific phage,eg. phage specific to bacillus cereus, then chloroform is used in a step for isolating these phages. Could there be any possibility that the...

23 April 2014 4,811 4 View