Thank you in advance.
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
Hello I'm using image registration over the bands of the micasense red-edge camera. Using RedEdge band as the fixed image. After removing inliers I can notice that inliers of the NIR band are...
13 January 2021 3,980 2 View
Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...
18 November 2020 3,867 3 View
Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....
18 November 2020 8,769 3 View
Hi there I'm planning on annealing two PCR products to perform some mutagenesis in a plasmid. I was recommended to try and keep it to 600pb each, for example, but for one of the plasmids there...
15 November 2020 8,656 3 View
Hello! I am in the process of writing my dissertation proposal and need to figure out sample size (I will be using G*Power). To measure my DV, I am using a survey that has 4 subscales each with a...
29 October 2020 8,609 2 View
In my current lab we have one cell culture room with 2 thermo biosafety cabinets facing each other. Some people in the lab just don't feel safe to use one hood if the other one has the UV light...
23 September 2020 5,379 2 View
Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...
21 September 2020 9,653 6 View
I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...
21 September 2020 5,213 6 View
In an experiment I found that the fresh weight of the root was less than that of the stem, however when I found the dry weight, the weight of the stem was less than that of the root. Why are these...
20 September 2020 2,269 16 View
during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...
22 February 2021 380 5 View
Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...
16 February 2021 2,007 3 View
I am working on one optimization problem. the model with two states, the transition between the two states based on one threshold value (L = 5, which L< 5 -> Normal state, L== 5 ->...
11 February 2021 4,045 3 View
i am trying to formulating one formulation but the problem i am facing is after formulating and adjusting it's pH, when i try to check pH after 7 days it is getting down towards the acidic side....
02 February 2021 9,343 3 View
Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...
25 January 2021 7,752 4 View
Hello everyone! I'm looking for some novel ways to measure out the amount of lead shot spray found in wildlife tissue. I have radio-graphed some deer ribs and gut piles left by hunters. They are...
24 January 2021 2,150 7 View
Hello everyone. I'm a pharmaceutical scientist whose experience is exclusively in solid dosage form. I'm starting a project that involves a semi-solid (cream) formulation, but I can't find a lot...
24 January 2021 4,055 3 View
Need to have a validated, stable method to separate tricholine citrate and cyproheptadine hydrochloride from a syrup formulation.
20 January 2021 4,216 2 View
The realistic approach to identify the exact choices and or preferences is quite difficult seems due to multiple unrealistic profiteering triggers applied to confuse and deviate the end-users from...
25 December 2020 8,304 1 View
I need to formulate the lagrangian dynamic equations for the hexapod robot's body. The body will constitute 6 DoF, which is 3 linear and 3 angular. I have the coordinates of each corner of the...
24 December 2020 1,222 1 View
