Starting from a z-stack of fluorescent images acquired imaging a 3D multicellular spheroid with a confocal or light-sheet microscope, with the single cells stained in the nucleous with DAPI, is there any tool for counting the total number of cells composing the spheroid?
https://www.unige.ch/medecine/bioimaging/files/3714/1208/5964/CellCounting.pdf
Yes, there is the Olympus Scientific Solutions automated cell counter Model R1. It uses an algorithm that is designed to work with liquid lens technology similar to the way human eye focuses on objects of interest. It is accurate and fast for counting a variety of cell types. The instrument is also portable and provides user-friendly touchscreen input and commands. It is expected to reduce your workload and standardize your cell counting process.
Hello, We have developed our own method for the detection and quantification of 3D multi-sphere cell culture in matrix.
- Badges
- Science topic