I have tried free trial version of FCS express software for analyzing DFI data. However, I am finding some technical difficulties using the same. That is why I have put forward this question. Thank You.
At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...
15 February 2021 9,145 2 View
The organo montmorillonite nanoclays modified with octadecylamine were used for the experiment. After acid treatment, the surface area will be increase. However, in my experiment, the surface area...
10 February 2021 1,581 3 View
Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...
05 February 2021 8,721 3 View
The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...
31 January 2021 729 6 View
Please share your experience on the effectiveness or challenges on online teaching during Covid-19 lock down. Share your experience about challenges, technology, online learning platform,...
26 January 2021 4,532 37 View
Recently I have generated LC-MS metabolomics data. After annotation, some have their compound name but either no KEGG id or KEGG id but no pathway. How to interpret those compounds? Additionally,...
18 January 2021 1,938 3 View
The current global pandemic, the climate crisis, the destruction of the oceans (with plastics, etc), and massive numbers of refugees are part of a set of serious global problems. It is clear...
13 January 2021 2,022 3 View
I want to remove a duplicate citation
01 January 2021 3,983 3 View
Hello, I am a newbie in geant4. I'm learning it playing with example. Sometimes, when I go to simulate an example code, it doesn't create an executable file (the green one in my ubuntu terminal)....
26 December 2020 9,482 3 View
Hello all, I want to do glycosaminoglycan (GAG) simulation in Gromacs. Initial structure and CHARMM topology for short chains can be obtained from Charmm GUI glycan modeler. But I have to take...
23 December 2020 6,092 2 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...
28 February 2021 2,338 1 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...
26 February 2021 9,580 4 View