Is there any simple alternative technique to GFP tagging?

Kulandaivelu Velmourougane @Kulandaivelu_Velmourougane

07 July 2016 0 8K Report

GFP tagging is most commonly used tehnique to visualise introduced microbes in rhizosphere. Is there any simple alternative technique to GFP tagging to study root colonisation?

Badges
Science topic

More Kulandaivelu Velmourougane's questions See All

Do living things other than human beings have sixth sense (reasoning capabilities)?

Humans have reasoning capacity. Do other living things on earth including microorganisms, insects, birds or animals have reasoning power?. Because, it is assumed that sixth sense separate humans...

09 October 2018 4,685 0 View

How to submit whole transcriptome RNAseq to NCBI?

We have done whole transcriptome sequencing of bacteria and fungi. Is there any simple tutorial to submit these NGS data to NCBI.

05 June 2017 8,362 6 View

How long can we store plant samples in Glutaraldehyde for SEM analysis?

I have to do SEM analysis of root samples colonized by a selected bacterium. After removal of roots from the pots, how long I can store the samples in 2.5% Glutaraldehyde solution. Is there any...

11 December 2016 1,900 7 View

How to store the extracted extracellular and intracellular polysaccharides?

In my biofilm study, i have extracted EPS and IPS. Different protocols are available on their storage (ultra pure water, buffers, distilled water, solvents etc.). Can anyone provide me simple...

06 July 2016 3,218 6 View

Can anyone help me in choosing correct statistical method to analyse the following data?

Experiment was conducted to study the effect of particle size, N factor and soil moisture on degradation of wheat straw treated with herbicide Main treatments: 1.Control (No...

11 December 2015 9,314 7 View

Can anyone help me to get GFP plasmid for root colonization study?

For my Ph.D (Microbial biofilms), i am going to use Azotobacter (Gram negative, N fixing bacteria) strain. I have to study the colonisation behavior of this bacteria in wheat rhizosphere. For this...

11 December 2015 9,743 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View