Do Inhibition of protein denaturation consider as a screening test for anti inflammatory activity of plants? If so why ?

Some people give an opinion that Inhibition of protein denaturation is not a relevant work and also cannot perform with plant extract. So like to get new views.

02 March 2019 1,839 9 View

Hi, which is the proper method for the inhibition of protein (albumin fraction) denaturation by plant extracts?

I am in doubt about different methods of preparations and calculations. While I performed it I got turbid material after denaturation by heating at 57 C for 3 minutes. So the absorbance at 416 nm...

08 September 2018 4,702 1 View

Can we use methanolic extract dissolved in dmso for biosynthesis of silver nano particles?

Aqueous extract is difficult to get from my plant. So I would like to try with other solvent extracts. These can dissolve only in dmso. So anybody can tell me any reaction of dmso or alcohol do...

07 August 2018 575 9 View

Is there any possibility to obtain the percentage of inhibition as above 100 after calculation?

i did my calculation for reducing power assay. Since the control value is very less than that of sample, i got % of inhibition as above 100.

02 March 2018 7,431 4 View

Whether we have to use different blanks for each concentration of extracts or standards for DPPH assays and other assays?

What has to use as blank for DPPH assay, nitric oxide scavenging assay, reducing power assay etc. Or what should be use as blank for antioxidant, anti inflammatory, antibacterial assays commonly...

01 February 2018 8,204 3 View

How to make serial dilutions or different concentrations of extracts dissolved in dmso or other solvents?

I would like to make different concenterations of my plant extract in different solvents such as 5ug, 25 ug,50 ug, 100 ug, 150ug etc per 1 ml for different assays.

01 February 2018 8,253 9 View

Is there any option to calculate total flavonoid content of my plant extracts in different solvents without using standard?

i have to know the total flavonoid content of my plant extracts in different solvents. But the standard is not available at present. So if any other options, please let me know. Thanking you in...

31 December 2017 3,264 6 View

Can anyone give the reason for getting less value for control in BSA denaturation assay?

I have done repeatedly BSA heat denaturation assay but I get more value for denatured one with sample and test than that of control. I measured in both 660 nm and 416 nm. It is not any coloured...

11 December 2017 1,970 3 View

Can anyone give an idea that whether difference in altitude and climate cause changes in plant's appearance, texture, internodal length etc.?

I have a plant which shows minute differences in different locations. This is having many species with similar properties.

10 November 2017 2,171 6 View

Can anybody give the exact absorbance (wavelength) for inhibition of protein denaturation?

I could see 660 nm and 416 nm in different papers. I have doubt that in this study whether we measure the amount of protein which is not denatured or some other content. If the absorbance gives...

10 November 2017 3,341 5 View

No title

02 March 2021 932 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View