I want to identify X- and Y- bearing spermatozoa subpopulations using flow cytometry machine. We want to use Acridine Orange staining for this purpose and I need the easiest protocol for this process if it's possible. Thank you all.
We run Fungal Panel using Molecular PCR Technique. We extract Nuclease Free water in the same way as we extract the sample. This eluted NFW is used as negative control on our PCR Plate for quality...
16 April 2024 776 1 View
In order to calculate CVI and CVR, the 4-points of Likert Scale frequently being adapted to determine the item validation. The indicators of 4-points used degree of relevance (1-not relevent,...
04 March 2024 2,838 2 View
I am looking for references to look for the criteria of experts groups before conducting pilot testing. The experts i believe will receive the questionnaires to review before the real study being...
25 February 2024 1,519 3 View
The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask...
11 December 2023 7,002 2 View
what is the most (internationly) accepted waste management guideline for veterinary hospitals and veterinary laboratories for medical waste in veterinary hospitals and laboratories, how can we...
24 July 2023 2,006 3 View
What makes usp A (universal stress protein) gene considered valid for molecular identification of Escherichia coli bacteria (used as PCR Primer)? Why use usp A while there are several groups of...
18 June 2023 4,022 2 View
How to dissolve plant ethanolic extract in sterile aquades if it is not dissolving completely? Should i dissolve it first using DMSO or ethanol in low conc before dilute it with aquades?
29 March 2023 2,769 1 View
Hi: I need any advanced Public Finance Reference Book for post-graduate studies. the reference with mathematical applications or methods. Regards
03 December 2022 4,396 5 View
We need to understand the relationship between two dependent variables (Frictional Noise and Coefficient of Friction) and three independent variables (Material Hardness, Surface Roughness, and...
12 July 2022 257 2 View
I have raster images from three models and I want to create their ensemble output. The correlation among rasters is near 70%. Is it a scientifically true approach? Can we combine 3 model outputs...
05 July 2022 5,345 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Purification of gst tagged protein
25 July 2024 1,720 0 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View