Nisa Demircan

5 Questions 5 Answers 0 Followers

Questions related from Nisa Demircan

Is there any recommended protocol for Hoechst 33342 dye to stain bull sperm imaging?

I want to stain bull sperm cells (dead/alive) with Hoechst 33342 (10 mg/mL in H2O) and don't know how to do it properly. I will be grateful if you could help me. Best regards and stay healthy.

21 October 2021 9,487 3 View

Is there any recommended protocol for bull sperm by flow cytometry machine?

I want to identify X- and Y- bearing spermatozoa subpopulations using flow cytometry machine. We want to use Acridine Orange staining for this purpose and I need the easiest protocol for this...

16 October 2021 1,785 0 View

Are there any differences between raw and frozen-thawed bull sperm prior to flow cytometric analysis?

I want to identify/sort X- and Y- bearing spermatozoa on frozen-thawed bull sperm samples using Hoechst staining by flow cytometry. Should I use raw semen before flow cytometric analysis or can I...

29 September 2021 9,096 3 View

What is the ideal concentration of acridine orange solution should be used in fluorescent microscopy for bull sperm analysis?

I want to determine bull sperm DNA integrity using ''Acridine orange hemi (zinc chloride) salt, Fluorescent cationic DNA and RNA dye '' of abcam's. Does anyone know an ideal procedure and...

14 August 2021 4,834 2 View

Cell-based approach ideas for the production of antibiotics?

I need to define two different strategies utilizing cell-based approach for the production of PVP-I (povidone iodine) and chitosan (a bioactive polymer) for my final project. Where should I start...

01 June 2021 7,829 2 View