How to dissolve plant ethanolic extract for seed germination assay?

30 March 2023 1 3K Report

How to dissolve plant ethanolic extract in sterile aquades if it is not dissolving completely? Should i dissolve it first using DMSO or ethanol in low conc before dilute it with aquades?

Andrew Paul McKenzie Pegman

Leave the mixture in an ultralow temperature freezer for 24 hours. The ethanol will separate the soluble components of the extract during this soaking period. Then apply aquades. Or simply remove the non-soluble elements by filtration since not all plant material will dissolve in ethanol :)

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