Hi there,
I have different samples in which I want to detect spinosad presence (qualitatively). I do not want to use HPLC and ELISA kit. Is there any other rapid method by which I can achieve it?
Hello, I think for true and reliable results you need HPLC or ELISA. HPLC methods follow a reasonably standard clean-up with HPLC determination relying on UV or MS detection to measure the individual spinosyns, and in residue trials provide data on spinosyns A, D, K, B and B of D. Spinosyn A contributes most of the residue most of the time and some HPLC methods have concentrated on spinosyns A and D since national authorities have decided that spinosyns A and D should constitute the definition of the residue. Immunoassay methods may or may not require a clean-up before the final colorimetric determination. The method is specific and reports the sum of the spinosyns. The HPLC and immunoassay methods have been extensively validated with numerous recoveries on a wide range of substrates and when used side-by-side in trials agreement was usually good.
Hello, I think for true and reliable results you need HPLC or ELISA. HPLC methods follow a reasonably standard clean-up with HPLC determination relying on UV or MS detection to measure the individual spinosyns, and in residue trials provide data on spinosyns A, D, K, B and B of D. Spinosyn A contributes most of the residue most of the time and some HPLC methods have concentrated on spinosyns A and D since national authorities have decided that spinosyns A and D should constitute the definition of the residue. Immunoassay methods may or may not require a clean-up before the final colorimetric determination. The method is specific and reports the sum of the spinosyns. The HPLC and immunoassay methods have been extensively validated with numerous recoveries on a wide range of substrates and when used side-by-side in trials agreement was usually good.
Karen A. Darbinyan Thank you for your views. I want to analyze a large number of samples, so for that reason i want to take a guess of spinosad presence in yes or no. Then for detail studies i will use HPLC. Yes, ELISA can be used for that reason but is there any alternative?
You may be able to use a spectroscopic method depending on the matrix of your samples and wether you have a pure sample of spinosad available as well as the concentration in target samples. If the matrix is known, you could use it as a blank and then use a spectroscopic method like FTIR or Raman and check for characteristic absorbance bands. At first glance this large organic molecule should absorb in both, however neither method is suitable for very small concentrations, especially when looking at an aqeous matrix.
Asad Naz If you have large number of samples so better choice is ELISA, there is no other technique which can offer more rapid analysis for large number of samples.
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