I was wonderring if we could see how nucleotide mutations would change DNA folding (e.g.by making some loops or intracions between neighbor sequences).
I have a fraction on my RNA blot less than100nt which I guess to be microRNA. I have no idea which kits are suitable for small RNA extraction. I got this RNA along with my main viral RNA using...
11 December 2013 3,758 8 View
I am trying to set up a southern blot assay for HBV core DNA detection from transfected cells. I extracted DNA using Immune precipitation of core particles followed by phenol chloroform - salt...
07 August 2013 6,792 11 View
I've got a windows 7 on my PC and can't install Gene Runner on it. I searched a lot but couldn't find a software which contains all the possibilities together. Does anyone have any suggestion?
06 July 2013 675 24 View
I use vectors containing whole genome of HBV for my transfections. To check viral replication, I get the supernatant of the cultured hepatocytes and concentrate the virus by centrifugation over...
31 December 2012 4,716 3 View
I am using my Viral RNA for Northern Blotting and every time I run my samples on Formaldehyde gel stained with Ethidium bromide I see no band not for my samples nor my RNA Marker. I am wondering...
31 December 2012 8,905 7 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View