Elham Shirvani Dastgerdi

6 Questions 11 Answers 0 Followers

Questions related from Elham Shirvani Dastgerdi

Can someone recommend the process needed for microRNA extraction and sequencing?

I have a fraction on my RNA blot less than100nt which I guess to be microRNA. I have no idea which kits are suitable for small RNA extraction. I got this RNA along with my main viral RNA using...

12 December 2013 3,742 8 View

Is there any program to model DNA with a given sequence?

I was wonderring if we could see how nucleotide mutations would change DNA folding (e.g.by making some loops or intracions between neighbor sequences).

09 September 2013 1,816 0 View

How important is DNA purity for a Southern blot?

I am trying to set up a southern blot assay for HBV core DNA detection from transfected cells. I extracted DNA using Immune precipitation of core particles followed by phenol chloroform - salt...

08 August 2013 6,769 11 View

Can someone recommend a software similar to Gene Runner, with such options as DNA translation in all frames, searching oligos, designing primers?

I've got a windows 7 on my PC and can't install Gene Runner on it. I searched a lot but couldn't find a software which contains all the possibilities together. Does anyone have any suggestion?

07 July 2013 586 24 View

I am using my Viral RNA for Northern Blotting and I see no band after running the gel.

I am using my Viral RNA for Northern Blotting and every time I run my samples on Formaldehyde gel stained with Ethidium bromide I see no band not for my samples nor my RNA Marker. I am wondering...

01 January 2013 8,887 7 View

Can plasmids pass throught the sucrose cushion along with viral particles?

I use vectors containing whole genome of HBV for my transfections. To check viral replication, I get the supernatant of the cultured hepatocytes and concentrate the virus by centrifugation over...

01 January 2013 4,695 3 View