Is their a known psuedogene for foxo3?

Hi Saeid,

If you go to http://genome.ucsc.edu/

Tool --> in silico PCR

And enter your primers, you will  see that they actually anneal to the FOXO3B pseudogene as Jack supposed.

There is indeed a problem with qRT-primers binding to gDNA. The reason is that, in case you have DNA contamination of your RNA you won't be able to differentiate the RNA signal from gDNA. That is also the reason why you should also perform a no-RT control of your samples (trying to amplify your RNA without performing the reverse transcriptase step).

For more information about good practice with quantitaive PCR I invite you to read the MiQe guidelines which is an exceptional piece of work to start with (in attachment).

Best,

Article The MIQE Guidelines: minimum Information for Publication of ...

thank you

that's very useful 

that"s interesting and useful ...thanks to Ludovic Barault