I wish to know if there is any chemical change due to repeated sterilization of buffer solutions.
The type of buffer and method of sterilization would influence the answer to this.
Yes
I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.
09 October 2017 6,393 0 View
As par of my research work, I have to deal with the expression patterns of certain genes. I came across different sequences for same genes in different publications. So can anyone please suggest...
10 November 2016 5,411 7 View
I am working on some fatty acids. I wish to know whether FABPs of Heart and Liver types are present in HL-60 cells.
08 September 2016 9,340 1 View
As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...
08 September 2016 5,252 4 View
As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.
07 August 2016 6,272 3 View
As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?
07 August 2016 3,108 2 View
I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...
06 July 2016 3,430 1 View
I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...
02 March 2016 6,640 1 View
Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.
11 December 2015 7,032 4 View
Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.
10 November 2015 8,325 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View