Protocol for in vitro cytokine stimulation of immunized CD8+ T cells?

Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...

02 December 2020 9,413 3 View

Why didn't PC3 CRISPR KI transfected with lipofactamine2000/3000 work?

27 March 2018 2,771 3 View

What is Ecological function?

Can anyone explain to me the meaning of ecological function? Is it Ecological function is same with Ecosystem function? their meaning is it the same?

08 January 2018 8,062 21 View

Why ligation failure and how to do

10 May 2017 8,645 5 View

Is it necessary to convert CFU/g to log (CFU +1)?

28 December 2016 8,661 5 View

Can RAPD be used for determining genetic similarity?

15 December 2016 1,289 3 View

No title

31 July 2016 6,292 7 View

CNS/supratentorial primitive neuroectodermal tumors (PNET) with cytokeratin and EMA expression?

The WHO definition of CNS PNETs is that: Tumors composed of undifferentiated or poorly differentiated neuroepihtelial cells which may display divergent differentiation alone neuronal, astrocytic...

04 May 2014 4,545 12 View

What are the decomposition products of dimethyl acetylenedicarboxylate in storage?

I recently purchased 3 bottles of dimethyl acetylenedicarboxylate from Sigma-Aldrich and decided to check the purity of the product using TLC. I found out that the chromatogram actually showed 2...

08 September 2013 7,019 6 View

Is there any method to determine the orientation of the 2-D molecule in a self-assembly layer on top of a solid substrate?

We are interested in understanding if a 2-D molecule is standing up or lying down on a surface.

24 January 2013 3,413 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View