We are currently identifying proteomic markers in different strains of M. tuberculosis isolated from sputum and CSF samples. Is there any other established protocol for eluting proteins apart from electroelution? Kindly let me know.
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During my field sampling, I collected one set of unfiltered groundwater samples i.e. acidified with a few drops of concentrated HCl. Can someone please help me how to prepare samples and get data...
19 November 2023 7,236 2 View
Is anyone familiar with the specifications of Silica (brand, catalogue number) is as good as silica packed in commercially available silica columns for DNA purification?
19 July 2023 5,107 0 View
(GitHub or installer)
12 July 2023 9,971 0 View
khaleid
20 May 2023 8,857 3 View
During my field study, I collected Groundwater samples and filtered them sequentially using 0.2um, 50kDa, and 3kDa. But when I analyzed these samples in the Shimadzu TOC analyzer, I got DOC...
21 March 2023 3,380 6 View
Toxicity of AgNPs
31 January 2023 9,170 0 View
I work on chromosomal aberration assay and sister chromatid exchange assay, some times I have after harvesting and staining, slides without metaphases or the nucleus isn't disrupted, or the...
29 January 2023 3,410 2 View
for how many times can I use/re-use Giemsa stain to stain my slides? and would that affect the tone of the stain and make it lighter?
11 January 2023 7,361 2 View
Microstructure : alpha ferrite matrix + bainite + martensite
17 November 2022 6,181 0 View
Hello Guys I have collected 9000 vitamin D samples and I want to know how to determine how many of the samples (having Vitamin D deficiency, Insufficiency, Normal, or toxicity). what I mean is how...
16 October 2022 9,732 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View