Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to write a program to print all the elements of a square matrix except the diagonal elements?

please include the code

12 September 2020 2,070 1 View

How can i write a c program to display elements of a two dimensional array which are divisible by 10 as well as their sum?

your explanation should include a workout as requested?

12 September 2020 3,151 2 View

How we can improve CfDNA concentration through extraction?

How we can improve CfDNA concentration through extraction?. Can we use Thermocycler or precipitation to concentrate CfDNA without damaging DNA integrity?.

19 May 2020 5,763 2 View

Problem in 3D dynamic meshing?

I am working on 3D dynamic meshing. The geometry is a rectangle in 2D and cuboid in 3D. The mesh is generated is tetrahedral. In 2D it is working fine. But in 3D I am facing a problem. Mesh...

28 April 2020 4,690 3 View

Which software can be used efficiently for sample size calculation in the biomedical research?

I am seeking for efficient and authenticated online/offline software or tool for sample size calculation in biomedical research.

08 August 2019 283 4 View

What are possible reasons/mistakes of amplification in PCR reactions having no tempelate i.e. NTC along with PCR reactions which have DNA. ???

I want to standardize detection of mutational changes in particular Genes by SSCP. I am using nested PCR for that but facing problems of weird amplification pattern in NTC reaction as well. I...

06 August 2019 2,811 10 View

Can anybody help me in tracing the availiblity of some published articles?

08 January 2019 3,634 2 View

How to solve MILP with Multiobjective functions in MATLAB/CPLEX Interface?

I have two objective functions based on a binary decision variable X (X takes either 0 or 1) - maximize A*X and minimize B*X. How to achieve that in MATLAB and CPLEX? Weights of each objective...

14 November 2018 4,267 2 View

Could r for non-parametric test be negative ?

Hello Dear All I have a SPSS data set of pre and post treatment of a group of patient and the data are not-normally distributed, for that I used (mann whittey U test) for p-value and for effect...

18 September 2018 10,004 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View