We used to change the giemsa solution once per month and the staining was always fine, however depending on what you are using it for (eg for use in clinical labs), you may want to perform a validation to confirm how often the solution should be changed by using the same solution for an extended period of time and assessing the quality of the staining. when it starts to deteriorate, that's when it should be ideally changed.

Hello Jordan, the Giemsa stain depends on the use and the frequency is how often you change it. I can tell you that once you have the working solution, its viability varies depending on the concentration you use (1:10 or 1:20). The truth is that after 30 to 50 slides or 15 days after its creation in aqueous formulation it tends to decay, I recommend you change the working solution at least every 15 days, maximum 21 if it is not exposed to light, then light It can degrade the azures in the solution. In particular, I would recommend wright giemsa but there is something left of each one.

If you need more information about wright giemsa do not hesitate to ask. Now consider that the more cellularity your sample has, the faster you will wear out the stain