I work on chromosomal aberration assay and sister chromatid exchange assay, some times I have after harvesting and staining, slides without metaphases or the nucleus isn't disrupted, or the chromosomes aren't spread on the slide. what can I do to fix that??
Is this problem with mutagen treated cultures or controls? There are many factors that can lead to this. Chromosome assays are beutiful methods but require lot of human expertise, experience, and guidance from the team as written protocols are not enough to establish high success rate and reproducibility. However, sharing following book, hope this helps.
You can refer to following book for excellent trouble shooting methods.
The AGT Cytogenetics Laboratory Manual, Fourth Edition
Editor(s):
Marilyn S. Arsham, Margaret J. Barch M.S., CG(ASCP)CM,, Helen J. Lawce B.S., CG(ASCP)CM,
First published:4 March 2017
Print ISBN:9781119061229 |Online ISBN:9781119061199 |DOI:10.1002/9781119061199
This is a big job! So you can't just give an answer. There are many unknowns: what object, what is the cause of aberrations, what methods were used to 1) influence the object, 2) what object (animal or plant cells), what mutagen, etc. You need to understand the subject. Your question is completely incomprehensible. What education do you have?
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