How to overcome the tension or frustration with others who are less organized or careless than you are?

Working with others usually good and build a massive amount of experiences, but sometimes there are some drawbacks of it. Careless, less organized, randomness, and commitment are some important...

30 October 2020 2,520 8 View

Tomato pollen internal membrane dye?

Hello everyone, I wonder if you can help to suggest any internal membrane dye that would enable me to stain the generative cell in tomato? unfortunately I have no marker tagged to the cell but I...

28 October 2020 2,335 3 View

Transient heat transfer through 2 walls and to air?

Dear All I am trying to simulate a problem that simplified as shown in the attached file. It has two different walls one inside the other and surrounded by air. I want to simulate the problem in...

14 October 2020 1,002 19 View

How many volume for media that we need to isolate exosomes from media?

Because i isolate 5 ml of culture media without serum and i got 1.2 ng miRNA . And i need 70 ng minimum that we can do PCR

28 September 2020 5,887 4 View

Is there any research points link Statistics and Big Data analytics that can be good points for research in PhD ?

Statistics and Big Data Analytics

02 August 2020 2,830 6 View

Which companies provide Molecular imprinted polymers ( MIPs) ?

19 July 2020 8,876 9 View

What are the differences between Renewable Energy and Energy Efficiency Technologies?

I found that energy efficiency deals with combustion efficiency, using biomass fuels and stuff like that. Can we link between wind energy and energy efficiency by some means?

07 June 2019 2,892 6 View

What are the best mesh quality criterion for inspection element quality?

I know about mesh density, skewness, wrap angles, and aspect ratio. Per literature, aspect ratio is a quick tool to define quality of mesh. Is there a rule of thumb for acceptable values of...

07 February 2019 7,145 4 View

Is Fmincon solver suitable for nonlinear ratio (I.e. NLP/NLP)?

I use fmincon to minimise weight to stiffness ratio. This ratio is Non linear. I tested this solver by using different initial points Xo. However, results are changeable and dependent on Xo. I am...

01 February 2019 3,069 3 View

What are the main considerations in injection mold designing and calculations?

I'd like to know the main parameters that should be considered in designing of injection mold. Also, I need to know a source for standardized calculations.

10 February 2018 6,412 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View