How to analyze the MD trajectories before and after enzyme-substrate binding?

I did MD simulation of enzyme-substrate complex using GROMOS96 43a1 force field for 1ns of about 500000 steps. I need to analyze the MD trajectories before and after enzyme-substrate binding. Is...

11 December 2017 4,892 4 View

What is the best method for MD simulation of best pose docked enzyme substrate complex?

I have doubts which I myself need to be clarified to setup the MD simulations more accurately 1. Is it possible to carryout MD simulations of best posed docked enzyme substrate complex? if so,...

11 December 2017 1,942 12 View

Does Fungi expresses endoinulinase and invertase at same time?

At an instance, does fungi produces both endoinulinase and invertase?

04 May 2017 8,676 0 View

What is the fate of the inulin in the culture media (broth) after autoclaving?

Will it retain its Degree of Polymerization or it degrades to shorter fragments

03 April 2016 1,623 3 View

I have 16S amplicon illumina data where barcode and primer sequences were removed; how to use this data for qiime analysis?

I have 16S amplicon illumina data where barcode and primer sequences were removed. How to use this data for qiime analysis?

11 December 2015 9,342 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View