Is there any method for the determination of beta carotene in carrot through spectrophotometer?

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Hicham Mechqoq

Dear Adnan Shakeel , the best way to analyze β-carotenes is by using HPLC, However, it is possible to do this using a spectrophotometer, check those papers. good luck

Agnishwar Girigoswami

Since it absorbs light around 450 nm (solvent dependent), definitely it can be analyzed by spectrophotometer.

Paul Milham

Check out the thread to the similar question asked by Philippa Ojimelukwe 5 years ago

Touseef Hussain

An Improved Method for Extraction of β-Carotene from Blakeslea trispora

February 2001
Applied Biochemistry and Biotechnology 90(1):37-45
DOI:
10.1385/ABAB:90:1:37