How can I calculate the specific growth rate and lag phase?

I am trying to calculate the specific growth rate for a species of Acetobacter. The substrate is glucose. Batch culture is used by regulating pH, temperature, and dissolved oxygen. According to...

02 March 2014 6,414 48 View

How can I determine that a cell population is under oxidative stress?

A bacterial strain has been grown under certain conditions, and catalase and superoxidase activity have been determined in exponential and stationary phase. During stationary phase, these enzyme...

08 September 2013 282 27 View

What is the Extinction coefficient that is used in the determination of enzyme activity ?

Could you please clearly explain the extinction coefficient and its importance in enzymatic assays? Should we consider it for all type of enzyme assays?

06 July 2013 1,002 8 View

What are the differences between cell aging and cell senescence?

Really, I would like to know the differences between these two terms from physiological point of view?

06 July 2013 8,895 25 View

Can I use cell crude extract to determine the effect of an inhibitory compound on one of the enzymes?

I have used French press to disrupt bacterial cells. After ultracentrifugation, the supernatant was used for determination of enzyme activity (PQQ-Glucose dehydrogenase). Now, I would like to...

06 July 2013 1,351 12 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View