Genetic code expansion (GCE) technology usually uses stop codon (e.g., TAG) to incorporate non-canonical amio acids into a protein. However, this might influence the expression of endogenous proteins by disturbing the translation terminiation.
Is there any research on this side effect of GCE? How would GCE influence the cellular homestasis and phenotype?
Genetic code expansion (GCE) is a technique that allows the incorporation of unnatural amino acids into proteins, expanding the chemical diversity and functionality of proteins beyond the 20 natural amino acids. GCE has been used for various applications, such as protein labeling, imaging, engineering, and therapeutics 1.
However, GCE may also have some off-target effects, such as interfering with the natural translation process, causing toxicity or immunogenicity, or altering the structure and function of the modified proteins. Therefore, it is important to evaluate the specificity and safety of GCE in different biological systems.
I searched the web for literature reporting the off-target effect of GCE technology and found some relevant articles. I summarized them in the attached table:
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