Ziqi Liu

4 Questions 1 Answers 0 Followers

Questions related from Ziqi Liu

How to perform high-quality immunofluorescence imaging for lysosomes in HEK293T cells?

Dear Community, I am investigating the subcellular localizations of some proteins in HEK293T cells and need to stain lysosomes as a spatial reference at the same time. Is there any good antibody...

26 April 2024 8,257 2 View

Is there any literature reporting the off-target effect of genetic code expansion (GCE) technology?

Genetic code expansion (GCE) technology usually uses stop codon (e.g., TAG) to incorporate non-canonical amio acids into a protein. However, this might influence the expression of endogenous...

15 August 2023 905 1 View

Is there any literature reporting NADH/NAD+ levels in different organelles or subcellular spaces?

24 March 2023 9,813 1 View

Why can pET17b vector lacking lac operator be induced by IPTG to express protein?

Dear Community, The pET-17b vetctor (the enclosed file) lacks the lac repressor expressing gene, and also seems to lack a lac operator in the upstream of the insert site. However, a lot of...

06 November 2022 1,027 1 View