Actually my CO2 incubator is damaged and I don't know the concentration of CO2 so my MCF7 cells are not reproduced actively. Basically I am using RPMI but it doesn't work good with low CO2. So is there a MEDIA that can work good in this condition?
Maureen Mee
You could add HEPES to the medium. That would maintain the pH, instead of the CO2 Bicarbonate
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Susan C Hubchak
L-15 media (Sigma L1518) supposedly can be used to grow cells without CO2, but better to get your incubator fixed:-) HEPES is used to prevent rapid shifts in pH when cells are out of a CO2 environment; not sure it would maintain pH in air, but worth a shot-try 25 mM.
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