How many mice are needed for long term potentiation?

As above, we are trying to do the Long-term potentiation test using mice hippocampal slices. How many mice are needed or how many slices are needed for LTP? Can anyone help me with that?

15 February 2020 7,073 3 View

Does anyone happen to know how to deal with kernel random matrix without using any expansion (i.e., Taylor, series, orthogonal or bi-orthogonal)?

The literature all depends on some kind of expansion. It is useful for global analysis but not for local.

28 January 2019 6,589 1 View

ARIMA, R square and stationary R square?

Dear all When ARIMA model was used to make the unstable time series data stable. There are many model to choose. which is the best one? Not only BIC value was shown, but also R square,...

24 December 2018 7,782 3 View

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

12 November 2018 3,755 4 View

Is there any textbook or technical instructions on the preparation of ceramic powder and sample?

Dear all, I am to do some experiment on ceramic powders, alumina for example and wondering how to prepare the powder and sample. I wish to have some recommendations on textbooks and technical...

04 March 2018 4,077 4 View

How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

31 October 2017 3,663 3 View

HOW TO DO ATRP REACTIONS ON THE SURFACE OF SiO2 NANOPARTICLES?

I encounter two questions during SI-ATRP. My experiment route is shown as follows. First ATRP reaction, MSN-Br(100mg), GMA( Glycidyl methacrylate, 1.5mmol),...

23 December 2016 3,066 3 View

Are deep neural networks practical to problems with low-dimensional input data(e.g. under 10 dimensions) ?

Deep neural network has been proved that efficient in imaging, text and voice data. Are they work good in processing low dimensional data ? (One example, using the DNN to analysis mechanical...

19 May 2016 3,367 3 View

How to do protein purification by intein tag/chitin beads in mammalian cells?

17 April 2016 1,304 2 View

What's the difference between the polarization direction, maximum radiation direction and direction of the electric field distribution?

10 February 2016 7,955 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View