Shib Shankar Saha

5 Questions 7 Answers 0 Followers

Questions related from Shib Shankar Saha

Can anyone suggest me a suitable growth media or technical tips for optimum growth of F. tularensis sub sp. holarctica LVS strain from stocked sample?

I have stocked my laboratory strain of Francisella (F. tularensis sub sp. holarctica LVS) in 100 % glycerol (800 microliter bacterial culture + 200 microliter 100% glycerol ) at -80C refrigerator....

06 July 2018 4,983 2 View

Is there any effect of frozen condition on mRNA for expression analysis of certain genes?

I want to check expression of certain genes of bacteria. But bacterial preparation with different treatment is taking long time. This is why i want to store bacterial pellet after centrifugation...

14 April 2017 3,144 3 View

Can anyone simply explain the principle of suicidal vectors?

I am planning to make mutant in Francisella novicida using suicidal vector. For designing primer set i need clear conception about principle of action of suicidal vectors. Can anybody send me the...

27 July 2016 1,005 2 View

Which plasmid is suitable for making complemented strain in intracellular bacteria?

Recently, I have made some mutant of known genes in Francisella tularensis. Developed mutants are showing its respective role. But during making complemented strain using pNVU plasmid, it could...

23 June 2016 6,781 2 View

"Liquid phage vs. solid phage hemolysis assay''- which one shows the critical differences?

I want to compare between WT and mutant strain of an intracellular bacteria. I need to know which type of hemolysis assay will show the maximum differences.

01 January 1970 161 3 View