5 Questions 7 Answers 0 Followers
Questions related from Shib Shankar Saha
I have stocked my laboratory strain of Francisella (F. tularensis sub sp. holarctica LVS) in 100 % glycerol (800 microliter bacterial culture + 200 microliter 100% glycerol ) at -80C refrigerator....
06 July 2018 4,982 2 View
I want to check expression of certain genes of bacteria. But bacterial preparation with different treatment is taking long time. This is why i want to store bacterial pellet after centrifugation...
14 April 2017 3,141 3 View
I am planning to make mutant in Francisella novicida using suicidal vector. For designing primer set i need clear conception about principle of action of suicidal vectors. Can anybody send me the...
27 July 2016 1,004 2 View
Recently, I have made some mutant of known genes in Francisella tularensis. Developed mutants are showing its respective role. But during making complemented strain using pNVU plasmid, it could...
23 June 2016 6,780 2 View
I want to compare between WT and mutant strain of an intracellular bacteria. I need to know which type of hemolysis assay will show the maximum differences.
01 January 1970 161 3 View