11 December 2020 2 8K Report

I want any datasets and appropriate algorithms/models that can be done with the dataset with respect to the construction industry. Mainly dealing with trains and bridges and building construction. Code also welcomed

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Scopus-indexed physiotherapy journal list?

Please List Scopus-indexed Open Access Journals with NO Publication Charges in Physiotherapy

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How to choose suitable buffer for liposome preperation?

I'm using LUVs as membrane models to study drug-membrane interaction. My composition of liposome is POPC : CHOL. I was confused about the selection of a buffer system for my liposomes. So far, I...

05 April 2023 6,803 3 View

What is the lipid composition of human heart (or cardiomyocyte) cell membranes?

I am working on membrane lipids and am entirely new to the field. Currently, I am focusing on heart cell membranes. I need the exact composition of lipids present in the human myocardial membrane....

24 March 2023 4,061 2 View

Proteinase K assay for assessing mitochondrial protein localization-what went wrong?

Hello everyone, I'm trying to perform Proteinase K assay for downstream analysis of the mitochondrial localization of a protein of interest. I isolated mitochondria from HeLa cells (using...

18 October 2022 2,106 4 View

Why is the 18S peak is very lower than 28S peak in total RNA pico bioanalyzer??

Our project is on transcriptomic sequencing of fish brain tissue at different salinity conditions. After isolating the total RNA, we did Bioanalyzer. We got no RIN value and the peak pattern was...

28 March 2022 6,304 1 View

Solution for Overset Mesh is moving outside from the overlap region in Star CCM++ while doing (PMM Test)?

I'm trying to do overset meshing in Planar Motion Mechanism (PMM) test in Star CCM+. while doing this I'm facing the issue that overset domain is moving outside from the overlap region. Thanks...

21 October 2021 1,133 5 View

Should human plasma (EDTA) be purified by filtration through a 0.22 micron filter (cellulose acetate) prior to exosome isolation?

I am looking to isolate exosomes from human plasma samples by a magnetic bead-based method. Is it recommended that I filter the plasma samples through a 0.22 micron cellulose acetate filter before...

15 June 2021 8,034 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 1,485 3 View

How to design a control feedback loop to control duty cycle for voltage regualation of AC to DC converter ?

I am working on a project in which I need to control the duty cycle of the pulse given to mosfet in an AC to DC converter.The mosfets are connected to LCL filter to give a regualted 5V.The output...

15 October 2020 6,442 6 View

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