I'm using LUVs as membrane models to study drug-membrane interaction. My composition of liposome is POPC : CHOL. I was confused about the selection of a buffer system for my liposomes. So far, I have tried PB and HEPES buffer. I am not getting good results on the DSC thermogram. Are there any guidelines for choosing a buffer depending on different experiments? If there, what are the compatible buffers for DSC and NMR studies of liposomes?
Kindly Help me with this. Any literature which gives a detailed analysis of liposomes with respect to buffer works fine.
Thank you!
Try tris buffer.
(PDF) Biophysical characterization of lutein or beta carotene-loaded cationic liposomes (researchgate.net)
You can also try purified deionized water to ensure your composition is OK and then try buffers. The phospholipid and cholesterol type, grade, and charge affect your liposomes also.
In reply to your question:
"How to choose suitable buffer for liposome preperation?"
Gokulakrishnan Manimaran
We can chose from a huge list of buffers or alternative hydration media based on the application we aim for.
From the Reference (listed below and attached):
" ....
Liposome hydration or suspension media can comprise any of the above-mentioned buffers or saline solution (0.9% w/v of NaCl in distilled water) or isotonic sucrose solution (9.25% w/v sucrose in distilled water).
...."
"... Alternative hydration mediums are saline or nonelectrolytes such as a sugar solution. For an in vivo preparation, physiological osmolality (290 mOsmol/kg) is recommended and can be achieved using 0.6% saline, 5% dextrose, or 10% sucrose solution (24). The aqueous medium can contain salts, chelating agents, stabilizers, cryo-protectants (e.g. glycerol) and the drug to be entrapped. ...."
Mozafari, M. R. (2010). Nanoliposomes: preparation and analysis. Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers, 29-50.
Nourhan S. Elkholy
Centrifuge your sample and collect the pellet and use for good results.
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