Is there any better antibody for glutathionylated proteins other than Clone D8?

More Sudarsan Rajan's questions See All

I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...

25 April 2024 9,798 0 View

How do i calculate cut off values in ELISA?

I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...

07 April 2024 3,079 1 View

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

I have selected two deep learning models CNN and sae for data analysis of a 1 d digitized data set. I need to justify choice of these two dl models in comparison to other dl and standard ml...

18 January 2024 6,800 1 View

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

I have used two dl models CNN and sae for data analysis of 1 d digitized data set. I need to justify use of these two models in comparison to other dl and other models. I am using ga to optimize...

18 January 2024 9,816 0 View

Which primers should I use for random amplification of bacteria from CSF sample?

I am working on 16s rRNA gene sequencing. So for that I should do PCR with random primers. Suggest me with a primer that I should use, also the which universal buffer the ti can use and if...

14 December 2023 6,288 0 View

(How) Is it feasible to use a questionnaire (of closed ended questions) to explore the lived experiences of participants in a phenomenological study?

Is it usual to use the closed ended questions in a Google sheet before carrying out semi-structured interviews for collecting data in a phenomenological study? If it is possible how can it be...

08 December 2023 2,290 7 View

Can anyone suggests a free software for finding particle size ?

For the measurement of particle size from XRD TEM SEM etc

12 November 2023 8,821 2 View

Is there any alternate way to avoid perfusion using a perfusion buffer for isolation of liver cells for primary culture?

Currently, I am working on mouse or rat models' primary culture of liver cells (particularly hepatocytes). In most of the previous research, I found the protocol stressing the perfusion of the...

10 October 2023 3,685 0 View

Can anybody provide me a reference about, TAHARA task which is based on the flow solver created by Prof. Yusuke Tahara of Osaka Prefecture University?

Required to understand how to solve potential flow around a ship hull.

29 September 2023 6,949 0 View

What percentage of scientific papers get more than 200 citations?

I know that many papers get 0 citations. I have read that 1.8% of papers get more than 100 citations. How about 200? What percentage of papers get 200 or 500 citations?

10 September 2023 5,973 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Nicholas E Hoffman

you can do mbcl confocal. super easy.

(E) HeLa cells were loaded with the reduced glutathione indicator monochlorobimane (mBCl) and imaged using a Zeiss LSM 510 with 405-nm excitation. (F) Quantitation of mBCl-GSH fluorescence. Data are mean ± SEM (n = 3). *p < 0.05, **p <

Sudarsan Rajan

Thanks Nick !