Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

No title

30 November 2020 5,089 5 View

Is there any recommended sampling size for case study in qualitative techniques?

Recently, I have attended a training where the instructor said 36 is the recommended sample size of case study in qualitative techniques. I am curious to know if the total population is less than...

14 March 2020 4,566 4 View

How to calculate the Width of the diagonal strut of infill wall with openings located at different location say doors and window?

Width of the Equivalent diagonal strut of infill masonry wall with Openings located at different position. Is there any formulas or need to be done in FEM Software?

03 January 2020 1,030 1 View

How to choose a perfect glass for solar still?

I need to select a transparent glass for solar still. but i know the specification all. but i need high transmissivity glass. but i dont know what brand , and what thickness i should choose. i...

13 November 2019 9,799 4 View

What will be the suitable medium to do a corrosion test for a aerospace grade Ti alloy?

Please list out some common corrosion takes place in the aerospace components and suggest me the possible medium to perform corrosion test for the aerospace-grade Ti alloy in a lab. Applications...

25 September 2019 9,161 3 View

How is research design related with and different from research methodology??

How is research design related with and different from research methodology??

11 June 2019 4,449 3 View

How to extract eigenvalues of the stiffness matrix in Abaqus PDE?

I am trying to obtain the eigenvalues of the global stiffness matrix in abaqus. I am able to extract the stiffness matrix using the input file and use MATLAB to obtain the eigenvalues. But I want...

03 June 2019 1,829 4 View

What is the maximum RMS current for GEIS that I can use for testing commercial 18650 batteries of capacities from 1 Ah to 3.3 Ah?

In order to obtain EIS results on commercial batteries of capacities from 1 Ah to 3.3 Ah batteries, what is the max RMS current that should be used in galvanostatic mode? I am using Versastat...

28 May 2019 2,165 5 View

What are all the Transcription factors (TFs) control the growth and development of insect pests?

Transcription factors (TFs)

02 May 2019 6,661 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View