Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

Where to add metamaterial unit cells in microstrip patch antenna to enhance the antenna parameters?

In order to enhance the antenna parameters, meta-material unit cells are added but where to load them is more advantageous either at ground plane or at the patch side, also is it necessary to add...

24 January 2021 2,999 4 View

How to represent Input data as multinomial distribution of feature vectors?

Hello everyone, Is there any approach to generate a fixed no. of feature vectors from the sequential data(similar to time series data...) and also express the same sequential data in terms of the...

29 November 2020 5,508 3 View

How to solve the HAZE problem in Low emissivity coatings ?

Dear friends, I am facing a problem with an issue of milky Haze in the coatings developed for aesthetic applications. All the layers were deposited by magnetron sputtering having thickness in...

08 November 2020 2,853 3 View

Lithium ion cell holder for Samsung 21700 - 50E cells?

Hello everyone, I have 5 Samsung 21700 - 50 E (NCM chemistry) cells. I want to do basic charge and discharge by connecting it in series. Does anyone know if there is a cell holder available...

05 October 2020 6,133 3 View

How the surface waves are linked to the dielectric constant of the substrate of an antenna?

How the surface waves can be reduced by choosing an appropriate substrate.

04 October 2020 8,609 3 View

What is the difference between PLSR and PLS-DA? Can we use PLSR for classification and prediction studies?

In all the textbooks related to chemometrics, PLS has been described as a regression technique that requires a dependent and independent variable for generating a regression line. However, in some...

17 August 2020 4,779 3 View

Nanotechnology in the field of nanofertilizers ?

I want to know the most important and productive role of nanotechnology in nano-fertilizers with some examples. Please add some knowledge to this topic. How much nanotechnology has grown in this...

08 August 2020 1,909 8 View

How to design double layer silver low emissive coating?

Dear friends of the research community, I am having an issue with double-layer low emissive coating fabrication. the coating of silver layer is not been adhere to the glass surface in this...

04 August 2020 7,730 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View