I measured the pH of bicarbonate solution by a pH-meter, and I also calculated the pH of the same solution using the quantitative relationship between pH and pOH. However, they differed a lot. Can I find a more accurate equation which relates bicarbonate concentration to pH ?
Hi Akin
Maybe you can try simulation systems like this one:
http://www.iq.usp.br/gutz/Curtipot_.html
should work in old and new excels
Dear Sir. Regarding your issue about the accurate equation which relates bicarbonate concentration to pH. These differences is also encountered in the Plasma bicarbonate concentration but can be solve by Henderson-Hasselbalch equation . Plasma bicarbonate concentration, a parameter generated during arterial blood gas analysis, is essential to the assessment of patient acid-base status. Blood gas analyzers do not have the capacity to directly measure bicarbonate; instead, it is calculated from measured pH and pCO2(a), using the Henderson-Hasselbalch equation that relates all three parameters. Bicarbonate is also one component of the much more frequently requested urea and electrolyte (U&E) chemical profile. In this case bicarbonate is directly measured as total carbon dioxide. Since patients who require blood gases will frequently also require U&E, it is common for there to be two contemporaneous bicarbonate results on the same patient, one calculated and one measured. Usually there is clinically acceptable agreement between the two values, but it is by no means rare for the two results to be wholly discordant with values differing by as much as 10-15 mmol/L. For more details see the following below link and the attached file which may help you in your analysis:
http://acutecaretesting.org/en/journal_scanner/journals/discordance-between-measured-and-calculated-bicarbonate-a-case-study
Thanks
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