Hello.
I am working with a diet induced obese model and run my mice on indirect calorimetry cages. I am search for the best method to adjust the energy expenditure (EE) results but there are some considerations to have in account.
It is assumed that fat does not importantly impact EE and people usually adjust EE to the lean mass (NMR based). However, my high fat diet (HFD) mice have 3 time more fat that the low fat diet (LFD) mice, and with it probably more brown fat also, that is very active metabolically. In this study is not completely correct to adjust neither by total body weight nor by lean mass, and absolute EE might be harder to analyze since BW differ a lot between LFD and HFD.
Is there any combined formula that is accepted to use? If so, can you reference the work?
Thank you very much.
Best regards.
Andre Cabral, PhD
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