Does anyone simulate the supercooling phenomenon of the PCM (COMSOL)?

I have a heat transfer model in the thermal bath (temperature changes in the range 23-33degC), in which a vial with PCM material is immersed (melting temperature is 28degC). During the heating...

03 January 2021 3,548 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View

Any suggestions to avoid the break of resin slices (Technovit 7100) on the microtome blade?

Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...

16 November 2020 2,373 3 View

How to obtain promoter sequences?

Hi dear fellows, I would like to perform ChIP-PCR on the promoter sequences of genes, but I am not quite sure how to design primers for the promoter sequences. Can someone please help me? My...

20 September 2020 7,371 6 View

Recommended packages or references to combine regional climate models and Holdrige life zones with R?

I want to use R to make regional climate models to analyze changes in Holdridge's life zones. I haven't used R in a long time so I'm kind of outdated and would like to read some references that...

01 July 2020 1,728 4 View

What strategies to use to make distance classes more attractive to students?

In times of pandemic, distance learning has gained strength. What can teachers do to make distance classes more attractive to students?

23 May 2020 8,148 22 View

In your opinion, are bibliographic review articles important and should they be published by scientific journals?

Some journals do not accept review papers because they consider that the theoretical framework is the basis of a research, and not the research itself. Other magazines already accept and encourage...

20 May 2020 7,841 50 View

Metabarcoding fungi in lake sediment cores - advantages/disadvantages of ITS and 18S?

In my PhD, I want to analyse fungal communities in lake sediment cores from Siberia. One of the performed experiments will be a metabarcoding of the fungi. Therefore I am evaluating primers at the...

05 May 2020 3,920 1 View

Which species do you recommend for ecotoxicological tests of industrial effluents containing hydrocarbons?

The question aims to know what types of species are most used in ecotoxicological tests of effluents from industries in the oil sector.

26 April 2020 5,382 21 View

How can I include a second order term in the model?

My hypothesis is that the functional diversity of fish (responsible variable) increases until an optimum level of a gradient of habitat structural complexity (predictor variable), but decreases...

01 April 2020 9,569 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

3D visualization of MRI images on a web browser?

Hello, would you have recommendations for the best technical solution (open code) to ensure 3D visualization of MRI slice images on a web browser? A solution that will allow users to rotate the...

10 February 2021 6,299 1 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How to calculate the acceleration/velocity of different molecules in an MD Simulation?

Hi, If I am performing an MD Simulation in which the two groups/molecules are moving in a direction on applying a force on the system, how would I calculate the velocities/acceleration of the two?...

08 February 2021 9,525 4 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View