How to calculate Leaf Water Conten Index (LWCI)?

I want to calculate Leaf Water Conten Index (LWCI) using Landsat or Sentinel data. I found several usefull formulas that use NIR and SWIR bands. But to estimate LWCI I have to have NIR and SWIR...

16 March 2020 9,322 4 View

Real Time PCR Analysis and Normalization?

If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...

18 December 2018 6,267 2 View

Gene Expression Analysis using R?

Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?

13 December 2018 5,140 5 View

Monoclonal antibody purity?

If monoclonal antibody would be obtained from ascites fluid during hybridoma expression in vivo -> protein A/G-sepharose purification, how much non-specific AB contamination and non-target...

04 December 2018 6,582 3 View

Does the plasmonic near field spread on the distance sufficient for the resonance light absortion (scattering)?

Cross section of light absorption and light scattering for noble metal (silver, gold) nanoparticle is higher than its geometrical cross section in case of plasmon resonance. It can be...

30 October 2018 5,235 3 View

Degenerate Primers to ITS Region (16S-23S rRNA) for NGS?

F: 50 -CTKTTGGGYYCCCKGRYYG-30 Rev: 50-CGCGTCCACTMTCCAGTTCTC-30 These are primers for NGS sequencing of bifidobacteria from a publication. If you substitute, for example K, with G or T then...

09 April 2018 6,351 3 View

How to obtain a precipitated powder of Poly-Amic Acid based on aromatic diamine and aromatic dianhydride?

How to obtain a precipitated powder of Poly-Amic Acid (PAA) based on aromatic diamine and aromatic dianhydride? It is well known that for several Aromatic Polyimides (PIs) in the course of...

22 December 2017 9,399 3 View

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film ?

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film

17 August 2017 1,331 1 View

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know?

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know? I know about stegoDB but can't find download link. Thanks

10 April 2017 8,150 9 View

Is that C. jejuni?

Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....

26 March 2017 3,470 12 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View