I am now subculturing the Raji cell line (ATCC), however, I am having trouble since after splitting the cell line for subculturing, I saw that the cells were in the dead phase even though the cell line was healthy before subculturing. Please offer me some advice on what to do.
Hello Md. Shahidul Islam
At what seeding density do you subculture?
You may split the culture when it reaches 2-3 x 10^6 viable cells/ml. Transfer cell suspension (in growth medium) to a centrifuge tube and spin at 1000rpm for 5minutes. Aspirate supernatant and add 10ml of fresh growth media to the cell pellet. Count the cells and transfer 0.5-5 x 10^5 viable cells/ml to a new culture flask.
Cultures can be maintained by addition of fresh medium or replacement of medium. But I would recommend that you subculture the cells using the optimal seeding density.
Best.
Here are some tips that may help you resolve the issue with your Raji cell line after subculturing:
If the issue persists, it may be helpful to consult with a cell culture specialist or a laboratory manager for further advice.
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