What should I choose for marking activated B cells?

02 March 2015 5,557 3 View

Are there any protocols for inducing immune tolerance to immunogenic protein in mice?

I am interested in immune tolerance. Are there any experimental protocols for incuding immune tolerance to an immunogenic protein? Thanks for your help!

07 August 2014 8,443 1 View

Why is chichen ovalbumin chosen as one of the most popular model antigen?

there are millions of proteins are immunogenic, however there are only several used as model antigens in immunological studies. One of the best known is chicken ovalbumin. Could someone tell me...

07 August 2014 7,893 11 View

Why is chicken ovalbumin chosen as one of the most popular model antigen?

there are millions of proteins are immunogenic, however there are only several used as model antigens in immunological studies. One of the best known is chicken ovalbumin. Could someone tell me...

07 August 2014 6,611 2 View

Do RAG mice have more NK cells than normal ones?

I am going to isolate murine NK cells. I see some research papers using RAG mice to isolate the cells. Why? Is it because there are more NK cells in the spleens of RAG mice? And what's the percentage?

11 December 2013 5,832 5 View

Is there any way of generating feeder cells other than irradiation?

I'm going to culture NK cells. Some protocols say irradiated autologous or allogeneic feeder cells are needed. However, we don't have irradiators. Does it mean I can't get the feeder cells I need?...

08 September 2013 973 1 View

Is it possible that Hydrocortisone turn into crystals after stock frozen due to the high concentration?

08 September 2013 4,290 2 View

Are there any standards for choosing suitable antibodies for panning?

I'm doing panning to deplete T&B cells with CD3/CD19 antibodies. CD19 antibodies can remove B cells effectively, however, hamster anti-mouse CD3e can barely adsorb T cells. Both antibodies were...

08 September 2013 9,123 6 View

Can I use a different cell lines as negative or positive controls when doing flow cytometry?

When doing Flow Cytometry, I used B16 cells as negative control of IFN-gamma and Granzyme B. But apparently, the sizes of B16 and lymphocytes are so different. Is this allowed? Or, I can only use...

04 May 2013 4,140 4 View

How can I culture purified NK cells in vitro for a long time, for example 1-2 weeks?

I'm culturing murine NK cells in vitro. However, the second day post isolation, 80% or more of the cells are dead or apoptotic. The medium I used was a-MEM supplemented with 10% FBS, 10 ng/ml...

03 April 2013 6,130 9 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View