NO pcr product in crispr clones?

I used 2 gRNA for knockout a specific gene. Isolated single clones.DNA extracted and run the pcr using diff set of primers (100 to 1000bp) either side flanking to target site but i don't see any...

07 June 2016 5,715 9 View

How to create Residue Topology File in CHARMM?

Hello All Can anyone help me with the simple tutorial(other than the common one available on the web) to construct a residue topology file for bimolecular interaction (Vander waal) in CHARMM....

24 February 2016 2,027 12 View

What are informatics tool to identify a new specific gene for conformation of micro organism?

Hi all I am working on food borne pathogens. now I want to design a PCR based techniques for the conformation of a specific organism. I know that, already there are so many genes in literature for...

22 April 2015 6,059 2 View

Can any one suggest the modelling, docking softwares other than Modeller anfd autodock?

i have alrady tried modeller 9.10 for modelling, but model generated from Modeller had large threads but ramchandran plot gives 97% allowed regions. can it be used for docking studies or not?

27 August 2012 728 0 View

I am facing problem in growing LNCap androgen sensitive prostate cells.Cells are not adhering to flask. Can anyone suggest me reg.this

I am growing LNCaP cell sfrom past 10 days . cells look round and healthy but not adhering to flask. Can any one help me in this reg

10 January 2012 1,603 3 View

Can anybody help me checking cytotoxicity of compounds in human macrophages (not cancerous)

Can anybody help me checking cytotoxicity of compounds in human macrophages (not cancerous)

19 October 2011 8,601 0 View

What should be the dimension of Agriculture Research in the wake of Covid-19?

Agriculture network (production-processing-marketing) is collapsed during lockdown due to Covid-19, monsoon is expected in 25 days, lot of preparation by farmers is pending, supply of inputs and...

01 January 1970 6,311 21 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View