How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to smooth the energy production peaks of PV and wind energy?

Is power-to-gas a possible solution to this issue? Storing renewable energy in the form of H2 and CH4 and using them as fuels can increase the penetration of renewable energy in the transportation...

28 October 2020 3,490 3 View

Which are the most promising kinds of feedstock for renewable fuels?

Which kinds of biomass, byproducts and waste are the most interesting for new research development in anaerobic digestion and gasification?

03 May 2020 1,434 25 View

Power-to-gas from renewable energy sources: pros and cons?

Based on your expertise, what are advantages and disadvantages of producing H2 or CH4 from renewable sources (wind, PV etc)? I am mainly interested in collecting different points of view, e.g....

28 April 2020 1,926 8 View

Where do I find the Wilcox H test?

Hi all, I need to compare some population means, but my data are non-normally distributed and with unequal variance (even after transformation) so I can use neither parametric t-tests nor...

10 February 2020 6,485 7 View

Biohydrogen and dark fermentation: what is their potential?

Is the conversion rate a limiting factor of this technology, compared to gasification and hydrogenotrophic/acetoclastic methanogenesis? And does hydrogen have the chance to become a successful...

06 January 2020 3,349 5 View

Hoe can you cut hislogical section cuntaining calcium fragments??

Hello everybody, I have to cut histological sections cuntaining calcium fragments. Obviously the section are perforated at the bone fragments and I can't recover row sections. Do you have any...

30 July 2019 4,829 1 View

Is there a standard procedure to pre-process continuous blood pressure data?

Is there any standard Matlab pipeline/toolbox to preprocess continuous blood pressure (BP) data? I have collected BP and ECG data simultaneously and would like to know which filters I should use,...

14 March 2019 4,888 3 View

Which is the best SSR primer set for varietal identification of T. monococcum and of T. aestivum? Are there any reference databases?

I'm looking for a panel of DNA primers for performing varietal identification of T. monococcum and T. aestivum. In addition I'm looking for a reference database of varieties and microsatellite values.

04 February 2019 5,053 2 View

Could someone help me in the identification of this barnacle (Crustacea, Cirripedia)?

02 November 2016 3,927 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View