Is there an equation available to calculate the molarity of guanidine isothiocyanate (GdnSCN) in solution over the refractive index? Like for Urea and GdnHCl.
Or is it actually the same equation for GdnSCN as for GdnHCl?
Equation for GdnHCl by Nozaki:
c = 57.147 (ΔN) + 38.68 (ΔN)² -- 91.6 (ΔN)³
The differences (ΔN) between the refractive index of a GdnHCl solution and that of water.
c = concentration of GuHCl solution in moles per liter.
Thank you.
All right, I found an equation. For everyone who is interested:
THERMODYNAMICS OF THE UNFOLDING OF a-LACTALBUMIN GUANIDINE THIOCYANATE
By K. TAKASE, K. NITTA and S. SUGAI (1974)
nD25 = 1,3327 + 0,02475 c
c = concentration of GdnSCN solution in moles per liter
nD25 = The refractive index at 589 nm and 25.0 °C,
The calculation for my GdnSCN stock seems legit.
- Badges
- Science topic
- Similar topics
- Linguistics
- Composition Studies
- Publication