What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Do I need to randomise samples in 96 well plate?

It's been recommended that I randomise samples across a 96 well plate, but not true randomisation, just don't run duplicate samples next to one another. Can someone point me in the direction of...

05 August 2020 7,510 3 View

What are the best emotional intelligence scales that contain subdimensions?

We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...

14 July 2020 7,122 2 View

Total of polyphenols in tea extracts conc mg/l to mg/g dry tea?

04 March 2020 3,649 7 View

No title

03 December 2019 5,253 1 View

How can I purify nanoparticles of PEG-PLA ?

Hello, I synthesize nanoparticles of PEG-PLA to encapsulate retinol. Once the nanoprecipitation process is completed, I would like to eliminate the unencapsulated active ingredient (retinol). In...

24 November 2019 8,853 2 View

What is the best research method for analysing documentaries?

I am in the middle of my assignment which is a small research project and I need to edit the methodology. My tutor has recommended me to find an hour long documentary online to analyse to do with...

29 July 2019 6,503 6 View

Purification of PEG-PLA ?

Hello, I'm working on PEG-PLA (5000:15000 Da) and I try to purify it after ROP polymerization. In different papers, they use purification by precipitation (usually with MeOH or diethyl ether)...

10 July 2019 386 9 View

Can I perform meta-analysis when the exposure veriable is continous?

I am hoping to undertake my first meta-analysis, where the exposure variable it mean number of decayed, missing or filled teeth)and the outcome is BMI category (underweight, normal weight,...

09 May 2019 8,757 4 View

Best method to amplify the signal in immunohistochemistry?

I am currently doing immunohistochemistry to look at a membrane antigen, on fixed embedded samples. I want to amplify the signal (low) I am getting, and was wondering which kind of amplification...

24 April 2019 5,263 5 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View