What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

When testing for outliers in a moderation analysis with a dichotomous moderator, is the overall sample size used to adjust Cook's and S.residuals?

This managed to throw me for a loop. When I'm assessing the presence of outliers before I conduct my moderation analysis I use SPSS to split the data file based upon the dichotomous moderator. I...

24 August 2020 6,578 3 View

Hello, does anyone have any links/resources for an extraction method of melatonin dissolved in AQ solution?

Hello, I currently have a product that has a small amount of melatonin dissolved in it (aqueous solution), and I need a sufficient amount to get a reading on an IR spec. I was thinking of...

04 December 2018 7,975 3 View

Does anyone have a protocol for the extraction of nuclear and cytoplasmic RNA from plant leaf tissue?

Hello, I'm looking for a protocol that will allow the extraction of nuclear and cytoplasmic RNA from the same plant leaf tissue sample (Arabidopsis thaliana). I have found protocols for the...

16 August 2018 158 2 View

Other than HPLC, is there a good method to measure intracellular retinoic acid (RA) concentrations in the cytosol of cultured cells?

I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...

10 October 2017 2,650 2 View

Does anyone know a good way of getting plasma from frozen whole blood?

11 July 2017 9,233 4 View

How to prevent background DIG staining in in situ PCR method?

I am performing in situ PCR of an RNA target in bovine lymph tissue; incorporating the DIG-dUTPs directly in the PCR product and then detection via anti-DIG Fab AP binding and subsequent...

08 February 2016 5,716 3 View

Are item means typically different in Xcalibre?

I am running an IRT analysis on an instrument in XCalibre, and the analysis reports substantially different means for the items than those calculated in Excel? Is there some weighting happening of...

30 January 2015 7,946 3 View

Is there a way to use immunohistochemistry to show changes in metabolism of murine muscle tissue following schema (glucose vs lipids etc)?

I am using the hind limb ischemia model in NOD/SCID mice and would like to measure changes in metabolism to the localised tissue both in a control mouse and mouse with recovered hind limb...

02 July 2014 7,096 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View