Are there good, affordable, sources of aggregation-prone Tau expressing cell lines?

Does anyone know of a good, commercial source for a cell line that expresses an aggregation prone Tau protein (e.g. P301L Tau), preferably in a well-characterized background (e.g. SY5Y). I don't...

01 February 2020 7,767 1 View

Why do manufacturers put countdown timers on rocking platforms?

I'm curious why a manufacturers put countdown timers on rocking platforms, and why users would ever want this feature. Specifically, timers whereby when the timer reaches zero the platform...

04 May 2019 9,703 2 View

How transferable are FPLC skills to HPLC?

I'm job hunting these days, and I have a lot of marketable experience using fast protein liquid chromatography (FPLC) for protein purification. However, many positions I'm applying to also prefer...

06 July 2016 3,652 6 View

Is there a good anti-NDRG1 antibody for use in mouse liver (or other tissues)?

I'm measuring p-NDRG1 and NDRG1 in mouse tissues. While I have good confidence in my p-NDRG1 antibody (Cell Signalling #5482), I have little confidence in my total NDRG1 antibody (Cell Signalling...

03 April 2016 8,281 1 View

What is the optimal polyacrimide concentration to separate p67 and p64 c-Myc by SDS-PAGE?

I want to resolve and separate p67 and p64 c-Myc protein by SDS-PAGE to recapitulate the below shown figure from the below linked paper. In the paper they used 10% polyacrylamide to separate p67...

02 March 2016 4,740 4 View

ATF4/CREB2 antibodies, different masses, which to trust?

Does anyone have an idea as to why two commercial ABs detect ATF4/CREB2 at different masses in identical samples, and which of these might be more trustworthy? I've used both Cell Signalling 11815...

01 February 2016 6,134 2 View

Loading Controls for Control vs Dwarf Mouse Livers? Discrepancy in Actin, Tubulin GAPD?

01 February 2016 6,642 5 View

Does anyone have a good NRF2 antibody for mouse fibroblasts and liver tissue?

I'm working on a project where I need to reliably quantify changes in NRF2 / NFE2L2 expression in mouse fibroblasts. Meanwhile, a colleague in my lab would like to measure NRF2 levels in mouse...

07 August 2015 7,960 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View