Brian Bower

9 Questions 163 Answers 0 Followers

Questions related from Brian Bower

Are there good, affordable, sources of aggregation-prone Tau expressing cell lines?

Does anyone know of a good, commercial source for a cell line that expresses an aggregation prone Tau protein (e.g. P301L Tau), preferably in a well-characterized background (e.g. SY5Y). I don't...

02 February 2020 7,875 1 View

Why do manufacturers put countdown timers on rocking platforms?

I'm curious why a manufacturers put countdown timers on rocking platforms, and why users would ever want this feature. Specifically, timers whereby when the timer reaches zero the platform...

05 May 2019 9,835 2 View

How transferable are FPLC skills to HPLC?

I'm job hunting these days, and I have a lot of marketable experience using fast protein liquid chromatography (FPLC) for protein purification. However, many positions I'm applying to also prefer...

07 July 2016 3,764 6 View

Is there a good anti-NDRG1 antibody for use in mouse liver (or other tissues)?

I'm measuring p-NDRG1 and NDRG1 in mouse tissues. While I have good confidence in my p-NDRG1 antibody (Cell Signalling #5482), I have little confidence in my total NDRG1 antibody (Cell Signalling...

04 April 2016 8,378 1 View

What is the optimal polyacrimide concentration to separate p67 and p64 c-Myc by SDS-PAGE?

I want to resolve and separate p67 and p64 c-Myc protein by SDS-PAGE to recapitulate the below shown figure from the below linked paper. In the paper they used 10% polyacrylamide to separate p67...

03 March 2016 4,843 4 View

Loading Controls for Control vs Dwarf Mouse Livers? Discrepancy in Actin, Tubulin GAPD?

I’ve detected a consistent discrepancy in the apparent expression of Beta Actin and Beta Tubulin in the livers of Snell dwarf mice and controls. This discrepancy exists even after ensuring that...

02 February 2016 6,744 5 View

ATF4/CREB2 antibodies, different masses, which to trust?

Does anyone have an idea as to why two commercial ABs detect ATF4/CREB2 at different masses in identical samples, and which of these might be more trustworthy? I've used both Cell Signalling 11815...

02 February 2016 6,267 2 View

Is there an easy way to measure the guanosine loading of GTPases?

I'm interested in checking the activity status of several GTPases (RHEB and the RAG GTPases) both in cells and possibly in tissues. It seems like the only methods to measure GTP vs GDP loading...

08 August 2015 8,571 1 View

Does anyone have a good NRF2 antibody for mouse fibroblasts and liver tissue?

I'm working on a project where I need to reliably quantify changes in NRF2 / NFE2L2 expression in mouse fibroblasts. Meanwhile, a colleague in my lab would like to measure NRF2 levels in mouse...

08 August 2015 8,059 3 View