Hello, I am planning to purchase Arabidopsis seeds from ABRC.
I found their catalog includes most genes in Arabidopsis.
Among them, many are T-DNA insertion lines from several massive projects.
Since I've never bought Arabidopsis mutant lines, I doubt whether the mutants really have
a non-functional gene which I want to see the functions.
I believe many researchers got their Arabidopsis from ABRC and left comments about it.
Is there some kind of archive for that???
I think not all donated mutants lines are bought and used for an experiment.
So some lines are never proven to have an appropriate mutation to knock-out the gene.
You are right that most of these lines are not characterized or verified. You could check the literature to see if anyone has published about any particular TDNA line & ask the authors for the seeds.
Most likely, you'll have to order several TDNA lines and characterize them. Just be aware that many seed lines don't contain the TDNA at all (cross-contamination during the initial screen). Also, many lines contain multiple insertions and about 20% have a TDNA-associated chromosomal translocation event. Just so you know what you're getting into. Independent lines & genetic rescue are the only real ways to verify the genotype --> phenotype connection.
Article Chromosomal translocations are a common phenomenon in Arabid...
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