As far as I know is better not to exceed 200 x 10^3/mL in the apheresis product but don't know if there is a low boundary and what the consequences for the cryopreservation success are.
When we undertake hematopoietic stem cell transplantation from a previously cryopreserved peripheral blood or a donor lymphochyte infusion we don't usually perform any additional flow cytometry...
08 September 2013 1,234 13 View
Median recovery of 52.3% (range 15.2–146.3%): These figures come from Bone Marrow Transplantation 25, 243–249 in the year 2000, so I guess improvements have been made. Does anyone have any more...
08 September 2013 7,926 3 View
When examining a possilbe confounding factor (Z) of the relationship of a 3-group categorical variable (X) (high-intermediate-low) with survival/relapse (Y) using the three classical criteria (KJ....
07 August 2013 1,743 1 View
I want to analyze (with "stcox") the overall survival outcome of a prognostic factor (varX), adjusting by a time-varying covariate such as stem cell transplantation. I assume it has to make a...
07 August 2013 9,332 10 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View